Journal: Journal of Cancer

Article Title: mRNA Expression of FGFR1 as Potential Marker for Predicting Prognosis of Surgical Resection of Small Cell Lung Cancer may be better than Protein Expression and Gene Amplification

doi: 10.7150/jca.44476

Figure Lengend Snippet: Diagrammatic representation of FGFR1 mutation from www.cbioportal.org .

Article Snippet: IHC for FGFR1 was performed using FGFR1 antibody (Cat, #BA0485, Boster Biological Technology Co. Ltd).

Techniques: Mutagenesis

Journal: Journal of Cancer

Article Title: mRNA Expression of FGFR1 as Potential Marker for Predicting Prognosis of Surgical Resection of Small Cell Lung Cancer may be better than Protein Expression and Gene Amplification

doi: 10.7150/jca.44476

Figure Lengend Snippet: FGFR1 protein expression by immunohistochemistry in SCLC and their correlations with prognosis. (A ) shows FGFR1 protein positive expression; ( B ) shows FGFR1 protein negative expression. Kaplan-Meier Survival analysis of FGFR1 protein-positive vs. FGFR1 protein-negative ( C and D ).

Article Snippet: IHC for FGFR1 was performed using FGFR1 antibody (Cat, #BA0485, Boster Biological Technology Co. Ltd).

Techniques: Expressing, Immunohistochemistry

Journal: Journal of Cancer

Article Title: mRNA Expression of FGFR1 as Potential Marker for Predicting Prognosis of Surgical Resection of Small Cell Lung Cancer may be better than Protein Expression and Gene Amplification

doi: 10.7150/jca.44476

Figure Lengend Snippet: Clinicopathological data of the patients with SCLC and FGFR1 status

Article Snippet: IHC for FGFR1 was performed using FGFR1 antibody (Cat, #BA0485, Boster Biological Technology Co. Ltd).

Techniques: Amplification

Journal: Journal of Cancer

Article Title: mRNA Expression of FGFR1 as Potential Marker for Predicting Prognosis of Surgical Resection of Small Cell Lung Cancer may be better than Protein Expression and Gene Amplification

doi: 10.7150/jca.44476

Figure Lengend Snippet: FGFR1 amplification by fluorescence in situ hybridization in SCLC and their correlations with prognosis. ( A ) shows FGFR1 amplification ( B ) shows FGFR1 non-amplification. Kaplan-Meier Survival analysis of FGFR1 amplified vs. non-amplified tumors ( C and D ).

Article Snippet: IHC for FGFR1 was performed using FGFR1 antibody (Cat, #BA0485, Boster Biological Technology Co. Ltd).

Techniques: Amplification, Fluorescence, In Situ Hybridization

Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

Article Title: Antagonist Antibodies to PD-1 and B7-H1 (PD-L1) in the Treatment of Advanced Human Cancer

doi: 10.1158/1078-0432.CCR-12-2063

Figure Lengend Snippet: PD-1, B7-H1, and B7-DC expression and prognostic significance in cancer patients

Article Snippet: , ( 58 ) , 150 , Paraffin IHC; anti-B7-H1 mAb (5H1) or anti- B7-H1 polyclonal Ab (4059, ProSci) , Membranous PD-L1 expression by melanocytes within the tumors had 3 patterns: no PD-L1; regional expression of PD-L1 on melanocytes colocalized with TILs (most common); and PD-L1 expression absence of TILs , Almost all PD-L1+ tumors were associated with TILs, while only 28% of PD-L1- tumors were associated with TILs; a sample of PD-L1+ tumors with TIL were shown to contain IFNγ, whereas no PD-L1- tumors examined contained IFNγ , PD-L1 was expressed on a proportion (57/150) of various MEL lesions, most commonly in close juxtaposition to TILs; when an inflammatory response to the tumor was detected, it was likely that both the tumor and infiltrating cells were PD-L1+; PD-L1 expression was associated with the superficial spreading and nodular MEL subtypes ( P =0.033) and not with MEL stage , Patients with PD-L1+ metastatic MEL (mMEL) had longer survival than those with PD-L1- metastatic MEL ( P = 0.032); patients with metastatic MEL with TILs had significantly improved survival compared with those without TILs ( P = 0.017).

Techniques: Expressing, Clone Assay, Membrane, Clinical Proteomics, Staining, Comparison, Functional Assay, Western Blot

Journal: Journal of Cell Science

Article Title: KazrinE is a desmosome-associated liprin that colocalises with acetylated microtubules

doi: 10.1242/jcs.047266

Figure Lengend Snippet: KazrinE gene structure and interaction with LAR. (A) Intron-exon organisation of the human kazrin gene. Arrows, translation initiation sites; TGA, translation termination codon; AATAAA, polyadenylation signal. Dashed arrow shows generation of kazrinE by alternative splicing. (B) Liprin phylogenetic tree. Dm, Drosophila melanogaster; Gg, Gallus gallus; Hs, Homo sapiens; Sc, Saccharomyces cerevisiae; Xl, Xenopus laevis. Sc_STE11 is included as a SAM-domain-containing outlier. (C) RT-PCR using primers to detect the kazrin isoforms shown or GAPDH as an input-level control. HK, human keratinocytes; –/–, no RNA. (D) Schematic diagram of kazrinA and kazrinE. KazrinE is shown with the kazrinA N-terminus (A). lz, leucine-zipper-like domain; nls, putative nuclear-localisation sequence; SAM, sterile α motif. The location of peptide immunogens is shown. (E) 293T cells transiently co-transfected with HA-kazrinE (KE) and GFP-LAR (LAR) were lysed in the presence (+PI) or absence (–PI) of phosphatase inhibitors and either examined directly by anti-HA immunoblotting (lysate) or following immunoprecipitation with anti-GFP (GFP IP). As a control, anti-GFP alone was added to beads (–). (F) A431 cells were transiently transfected with GFP (left-hand lane) or HA-kazrinE, serum starved for 16 hours and either left untreated (–) or treated with EGF (+) for 1 hour prior to lysis in the presence of phosphatase inhibitors. Upper panels: HA-kazrinE was immunoprecipitated and immunoblotted with anti-phosphotyrosine (p-Tyr) or anti-HA. Arrow indicates phosphorylated kazrinE. Lower panels: western blots of total-cell lysates probed with antibodies to HA or α-tubulin (α-Tub) as controls for transfection and loading, respectively. (G) Keratinocytes retrovirally transduced with HA-kazrinE were transiently transfected with GFP or GFP-LAR (green), immunolabelled with anti-HA antibody (red) and counterstained with DAPI (blue). The boxed region is shown at higher magnification in the right-hand panel. Scale bar: 10 μm.

Article Snippet: Exons 9 to 15 encode three sterile α motif (SAM) domains, which form a liprin-homology domain (LHD), the defining characteristic of liprins ( Stryker and Johnson, 2007 ).

Techniques: Alternative Splicing, Reverse Transcription Polymerase Chain Reaction, Control, Sequencing, Sterility, Transfection, Western Blot, Immunoprecipitation, Lysis, Transduction